To increase the performance of PMCA, 6 mM EDTA and 0.05 percent digitonin were added to the brain homogenate. Urine samples, processed as described above, were blended with 10 percent mind homogenate in tubes containing 3 polytetrafluoroethylene beads and subjected to 96 cycles of PMCA by using a microsonicator . After one round of 96 PMCA cycles, an aliquot of the amplified material was diluted by one factor of 10 into fresh human brain homogenate from transgenic mice and an additional round of PMCA cycles was performed. In order to avoid sample cross-contamination between rounds, thorough decontamination of instruments and products was performed by using 2 N sodium hydroxide or 4 M guanidine isothiocyanate.With its current profile, including impressive viral load decrease and good safety and tolerability profile, we continue to think that ACH-1625 has the capacity to become a best-in-class protease inhibitor for HCV treatment. ‘With this NS5A inhibitor, ACH-2928, as well as our high-potency, pan-genotypic HCV protease inhibitor, ACH-2684, also inside our pipeline of clinical candidates, we believe Achillion can be well positioned to take part in the huge and important HCV market.’ Fourth quarter results The Company reported a net loss of $6.2 million for the three months ended December 31, 2010, in comparison to a net lack of $6.december 31 7 million for the three months ended, 2009.1 million in the fourth quarter of 2010, compared to $4.9 million for the same period of 2009.2 million, in comparison to $1.9 million in the same period in ’09 2009.5 million, compared to a net loss of $25.9 million in ’09 2009.december 31 4 million for the year ended, 2010, consisting of QTDP program grants substantially, in comparison to negative $294,000 for the entire year ended December 31, 2009.5 million, compared to $18.4 million in ’09 2009.